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10× nh 4 buffer  (Meridian Bioscience)

 
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    Meridian Bioscience 10× nh 4 buffer
    10× Nh 4 Buffer, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10%C3%97+nh+4+buffer/10%C3%97+nh4+reaction+buffer/pmc03012678-71-19-22
    Average 90 stars, based on 1 article reviews
    10× nh 4 buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Barcoding rotifer biodiversity in Mediterranean ponds using diapausing egg banks
    Article Snippet: Each 50 μl PCR contained 5 μl of template DNA, 2 mM of MgCl 2 , 0.2 mM of each nucleotide, 0.2 μM of each primer, 5 μl of 10× NH 4 Bioline buffer, and 0.5 U of BioTaq DNA polymerase (Bioline).

    Polymerase Chain Reaction:

    Article Title: Upregulating CXCR4 in Human Fetal Mesenchymal Stem Cells Enhances Engraftment and Bone Mechanics in a Mouse Model of Osteogenesis Imperfecta
    Article Snippet: .. The PCR mix (20 μl per sample) consisted of 12.7 μl diethylpyrocarbonate (DEPC)-treated water (Invitrogen), 2 μl 10× NH 4 buffer (Bioline, Tauton, MA, http://www.bioline.com ), 0.6 μl 50 mM MgCl 2 (Bioline), 2 μl 2.5 mM dNTP (Promega), 20 μM each of forward and reverse primers as described elsewhere (5′-ACGTCAGTGAGGCAGATG-3′; 5′-GATGACTGTGGTCTTGAG-3′) [ 14 ], 0.2 μl BioTaq DNA polymerase (Bioline), and 2 μl cDNA. .. The reaction was run in a Peltier Thermal cycler (MJ Research, St. Bruno, Quebec, Canada, http://www.mj-research.com/ ).

    Article Title: Production of haploids and doubled haploids in oil palm
    Article Snippet: .. 10 μl PCR mixes comprised 1.0 μl 10× NH 4 buffer (Bioline), 0.3 μl MgCl 2 (10 mM), 0.4 μl dNTPs (10 mM), 0.2 μl each primer (10 mM), 1-5 ng DNA and 1U Taq polymerase (Bioline). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Promiscuous Expression of α-Tubulin II in Maturing Male and Female Plasmodium falciparum Gametocytes
    Article Snippet: .. The master mix for the RT-PCR was made with the following per 25 μl reaction: 2.5 μl of 10× NH 4 buffer (Bioline), 0.5 μl of 50 mM MgCl 2 (Bioline), 0.5 μl of 5 μM dNTPs (Promega), 1 μl of 5 μM of the chosen forward and reverse primers, 25 units of Taq polymerase (Bioline) and 3–5 μl of cDNA. .. Nuclease free water (Promega) was used to bring the reaction to a volume of 25 μl.



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